Callus culture of Cassia occidentalis L. was studied using nodal explants. Maximum callus induction was obtained
on basal MS medium supplemented with 2, 4-D (5 mg/l) and Kinetin (0.02 mg/l). Sigmoid pattern of growth was
obtained with highest growth index was 6th week and lowest at 2nd week of subculture. Analysis of plant parts revealed
maximum content of flavonoids in leaves of C. occidentalis (1.95 mg/gdw) and minimum in stem (1.24 mg/gdw). Callus
culture of C. occidentalis showed more total flavonoid content (2.01 mg/gdw) than the plant parts. Plant parts and in vitro
cultures were studied for their antioxidant potential using DPPH assay. Among the plant parts the highest activity was
found to be of leaves and lowest in seeds. The antioxidant potential of in vitro grown callus (IC50-155 µg/ml) was almost
at par with leaves (IC50-150 µg/ml).
Keywords: Antioxidant, callus culture, Cassia occidentalis, flavonoids.
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