Human umbilical cord blood (hUCB) is a primitive source of stem cells and is being banked worldwide for the
purpose of future clinical use. Lineage negative (Lin-ve) stem cells are one of the primitive populations in hUCB which
are capable of self-renewal and differentiation into multiple lineages. These cells have not been adequately investigated
for their stem cell characteristics. We have analyzed the immunophenotypic expression of CD45, CD34 and CD117
markers on three different cell populations in hUCB viz., mononucleated cells (MNCs), Lineage positive (Lin+ve) and
Lin-ve cells. The CD34 and CD117 expression was found to be 60 fold more in the Lin-ve population as compared to
MNCs and Lin+ve cells. This expression was further enhanced in CD45+Lin-ve fraction. The CD34+ and CD117+ Lin-ve
cells showed 99% positivity for CD45. Our data suggest that CD34+ CD117+ Lin-ve cells are reasonably enriched stem
cell population in hUCB which can be clinically used for transplantation and other therapeutic purposes.
Keywords: CD117, CD34, haematopoietic, lineage, stem cell, umbilical cord blood.
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