Title:Formulation and Pharmacodynamic Evaluation of Glibenclamide Incorporated Niosomal Gel
VOLUME: 11 ISSUE: 3
Author(s):Harini C. Vadlamudi, Prasanna R. Yalavarthi, Rao P. Satyanarayana, Vandana KR, Jayasri Vulava and Thulasi Chowdary Gutta
Affiliation:Department of Pharmaceutics, Sree Vidyanikethan College of Pharmacy, Tirupati- 517102, India.
Keywords:Blood glucose levels, Carbopol 934, diffusion, flux, glucometer, permeability.
Abstract:Objective: The research aims to formulate and develop the controlled release profile of glibenclamide by encapsulating
glibenclamide into niosomes followed by incorporation into an aqueous gel base. Materials and methods:
Glibenclamide incorporated niosomes were prepared by a modified ether injection technique using Span 20/Span 80 and
cholesterol. The prepared niosomes were evaluated for chemical incompatibility by FT-IR, morphology, vesicle dimension,
encapsulation efficiency, in-vitro diffusion and drug release kinetics. Niosomal gels were prepared by incorporating
the optimized niosomes into a gel base containing Carbopol 934 and evaluated for viscosity, in-vitro diffusion and in-vivo
pharmacodynamic activity. Results and discussion: The results indicated that relationship between the amount of Span
and niosomal vesicular diameter was inversely proportional. Microscopic images have illustrated the sphere shape vesicles.
The cumulative percentage of drug release from niosomal suspension was observed in the order GN-4>GN-2>GN-
6>GN-5>GN-3>GN-1. Glibenclamide gel showed highest percentage drug release when compared to niosomal gel. Invivo
study revealed that the glibenclamide incorporated niosomal gel formulation; GNG-1 is more efficient in lowering
blood glucose levels in experimental animals. Conclusion: The niosomal gel of glibenclamide had released the drug in
well controlled manner which is supported by pharmacodynamic activity with evidence of consistent lowering of blood
glucose levels.
