Amphotericin B (Amb) is a broad-spectrum polyene macrolide antibiotic which has been a drug of choice for
fatal conditions like fungal splenic abscess. Hence a drug delivery system with splenotropic potencial might be of great
significance for further therapeutic effectiveness. Present manuscript proposes development of Irregular shaped polymer
lipid hybrid nanostructures (LIPOMER) of Amb with enhanced splenic uptake. Amb LIPOMER (~400 nm) was prepared
using poly(methylvinylether-co-maleic anhydride) [Gantrez® AN119] and glyceryl monosterarate (GMS) as matrix material.
Conditions for preparation of formulation were optimized using 23 factorial design and effect of the concentration of
excipients on the particle size and entrapment efficiency was evaluated. Optimized formulation was further characterized
using Transmission electron microscopy (TEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and
Fourier transformed infrared spectroscopy (FTIR) techniques. Amb LIPOMER exhibited acceptable haemolysis when assessed
for In-vitro hemolytic potential in comparison to Fungisome TM. The high splenic uptake of Amb LIPOMER was
verified using gamma scintigraphic technique in small animal model. Results of our study indicated that LIPOMER system
can be a smart drug delivery platform for Amb to eradicate splenic infections effectively.
Keywords: Amphotericin B, enhanced splenic uptake, LIPOMER, nano-system, scintigraphy, splenic abscess.
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