The aim of the study was to evaluate antibacterial and antioxidant potential of the actinomycete isolate
Streptomyces cinnamonensis VITNS1 isolated from paddy field, Vellore, Tamil Nadu. The antibacterial activity of crude
extract was tested against bacterial pathogens. The free radical scavenging potential of the crude extract was determined
by DPPH assay. The morphological, physiological and the biochemical properties of the strain VITNS1 was confirmed by
conventional methods and genotypic characterization was carried out using 16S rDNA partial gene sequencing. The
authenticity of the crude chemical constitutes were determined by (GC-MS). The strain VITNS1 was identified as Streptomyces
sp on biochemical and morphological characterization. Phylogenetic tree was constructed and the 16S rRNA gene
sequence showed 99% similarity to Streptomyces cinnamonensis strain N56-1. The antibacterial activity studied against
Staphylococcus aureus (26mm) was found to exhibit the best spectra of activity, followed by Pseudomonas aeruginosa
(25mm), Salmonella typhi (25mm) Escherichia coli (24mm) at 30mg/mL concentration. The ethyl acetate extract possessed
DPPH free radical scavenging activity at 5mg/mL with 70% inhibition. The (GC-MS) Gas Chromatography revealed
the presence of six volatile compounds. Hence the results of antibacterial and antioxidant activity of the extract
clearly indicated that the terrestrial environment are the major sources for the isolation of bioactive compounds from
actinomycetes and a promising tool for drug discovery.
Keywords: Antibacterial activity, antioxidants, bioactive compounds, soil actinomycetes.
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