A novel and convenient strategy for iodine labeled glycopeptide molecular probe and purification was developed.
The fluorine rich bi-functional coupling agent, 4-tris(2-perfluorohexylethyl)stannylbenzoate succinimidyl ester, was
successfully synthesized via 5 steps starting from the fluorous Grignard reagent. It was purified by a simple and fast isolation
using perfluorinated hexanes (FC-72). The “cold” iodine labeled yield for the coupling agent was as high as 92%
within 15 min. The iodine-labeled product was only in organic fractions as we expected. It was shown that there was only
one major peak in organic fractions according to HPLC. Finally, the iodine-labeled coupling agent was applied to label
glycopeptide and afforded a high yield of 87% within 30 min.