Laccase Inhibiting Activity of Some Coumarin Derivatives

Author(s): Marina Tisma, Maja Molnar, Marija  Skarica, Milan Cacic, Bruno  Zelic

Journal Name: Letters in Organic Chemistry

Volume 11 , Issue 8 , 2014

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Graphical Abstract:


Seven coumarin derivatives were screened for their inhibitory effect on laccase utilizing ABTS and L-DOPA as substrates. 2-[(4-Methyl-2-oxo-2H-1-benzopyran-7-yl)oxy]acetic acid (E)-2-[(dihydroxyphenyl)methylene]hydrazides were proven to be stronger inhibitors than corresponding thiosemicarbazides when ABTS [2,2'-azinobis(3- ethylbenzothiazoline-6-sulfonic acid)] was used as a substrate. In kinetic experiments mixed type inhibition was determined for 2-[(4-methyl-2-oxo-2H-1-benzopyran-7-yl)oxy]acetic acid (E)-2-[(2,5-dihydroxyphenyl)methylene]hydrazide and (E)-2-[(3,4-dihydroxyphenyl)methylene]hydrazide. In reaction of L-DOPA (L-3,4-dihydroxyphenylalanine) oxidation catalyzed by laccase none of the tested compounds has shown inhibitory effect.

Keywords: Coumarins, laccase activity, inhibition, ABTS, L-DOPA.

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Article Details

Year: 2014
Published on: 13 June, 2014
Page: [583 - 589]
Pages: 7
DOI: 10.2174/157017861108140613161919
Price: $65

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