Caspases and ROS - Dependent Mechanism of Action Mediated by Combination of WP 631 and Epothilone B

Author(s): Aneta Rogalska, Barbara Bukowska, Agnieszka Marczak

Journal Name: Anti-Cancer Agents in Medicinal Chemistry
(Formerly Current Medicinal Chemistry - Anti-Cancer Agents)

Volume 14 , Issue 9 , 2014

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Graphical Abstract:


In this article, the synergistic effects of WP 631 and epothilone B (Epo B) combination in human ovarian cancer cells (SKOV-3) cells are investigated and the reasons for the exact mechanisms of action of both drugs co-administered are explained. Compared with single drugs, the combination treatment significantly enhances apoptosis as confirmed by increases in caspases (-8, -9, -3) activity, ROS level and DNA damage and decreases in mitochondrial membrane potential. The combination of the compounds activated both caspase - 8 and -9, indicates that both pathways of apoptosis, extrinsic (induced by the effect of Epo B) and intrinsic (triggered mainly by WP 631) participate in the proposed treatment. Thus, the results of this study strongly suggest a synergistic action of the combined treatment with WP 631 and Epo B in SKOV-3 cells death induction.

Keywords: Apoptosis, epothilone B, ovarian cancer, synergism, WP 631.

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Article Details

Year: 2014
Published on: 09 October, 2014
Page: [1261 - 1270]
Pages: 10
DOI: 10.2174/1871520614666140608150807
Price: $65

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