Title:Caspases and ROS - Dependent Mechanism of Action Mediated by Combination of WP 631 and Epothilone B
VOLUME: 14 ISSUE: 9
Author(s):Aneta Rogalska, Barbara Bukowska and Agnieszka Marczak
Affiliation:Department of Thermo biology, Institute of Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Keywords:Apoptosis, epothilone B, ovarian cancer, synergism, WP 631.
Abstract:In this article, the synergistic effects of WP 631 and epothilone B (Epo B) combination in human ovarian cancer cells (SKOV-3)
cells are investigated and the reasons for the exact mechanisms of action of both drugs co-administered are explained. Compared with
single drugs, the combination treatment significantly enhances apoptosis as confirmed by increases in caspases (-8, -9, -3) activity, ROS
level and DNA damage and decreases in mitochondrial membrane potential. The combination of the compounds activated both caspase -
8 and -9, indicates that both pathways of apoptosis, extrinsic (induced by the effect of Epo B) and intrinsic (triggered mainly by WP 631)
participate in the proposed treatment. Thus, the results of this study strongly suggest a synergistic action of the combined treatment with
WP 631 and Epo B in SKOV-3 cells death induction.
