Analytical Methods for Determination of Reactive Oxygen Species

Author(s): Joanna Pranczk, Dagmara Jacewicz, Dariusz Wyrzykowski, Lech Chmurzynski

Journal Name: Current Pharmaceutical Analysis

Volume 10 , Issue 4 , 2014

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Graphical Abstract:


This review focuses on analytical methods for the determination of reactive oxygen species (ROS): a superoxide/ hydroperoxyl radical, hydrogen peroxide, singlet oxygen and a hydroxyl radical. The methods which are described in this paper are based on: electron paramagnetic resonance spectroscopy (EPR), nuclear magnetic resonance (NMR), mass spectroscopy (MS), spectroscopy UV-Vis, gas chromatography (GC), chemiluminescence (CL), fluorescence (FL), HPLC –UV, iodide titration, and 14C-formate oxidation. In this review, the most common analytical methods for the detection of ROS generated by chemical methods are presented. The methods are based on the direct as well as indirect detection of ROS.

Keywords: Analytical methods, reactive oxygen species, radicals.

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Article Details

Year: 2014
Published on: 03 September, 2014
Page: [293 - 304]
Pages: 12
DOI: 10.2174/1573412910666140530221254
Price: $65

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PDF: 57