In this study, we aimed at the synthesis of new 2-((5-substituted-4-methylthiazol-2-yl)amino)-2-oxoethyl 4-
substitutedpiperazine-1-carbodithioate derivatives and their antibacterial, antifungal, antioxidant and AChE inhibitory
evaluations. A set of fifteen new compounds of 2-((5-substituted-4-methylthiazol-2-yl)amino)-2-oxoethyl 4-substitutedpiperazine-
1-carbodithioate derivatives were synthesised by reacting 2-chloro-N-(5-substituted-4-methylthiazole-2-
yl)acetamide derivatives and sodium salts of appropriate N-substitutedpiperazine dithiocarbamic acids in acetone. The
chemical structures of the compounds were elucidated by IR, 1H-NMR, 13C-NMR, MS spectral data and elemental analysis.
The antibacterial and antifungal activities of the synthesised derivatives were tested against Gram (+), Gram (-) bacteria
and yeasts such as Salmonella typhimurium, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli,
Candida albicans, Candida utilis, Candida tropicalis, Candida krusei and Candida glabrata using a microbroth dilution
technique. Antimicrobial results showed that the compounds displayed minimum inhibitory concentrations in the range of
15.62-4000 µg/mL. Antioxidant activity of the synthesised derivatives was measured by scavenging activity against
DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical and qualitative ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid)) free radical scavenging assay and it was found that the compounds showed antioxidant activity. AChE inhibitory
activity of the synthesised derivatives was tested against Donepezil for their ability to inhibit acetylcholinesterase (AChE)
using a modification of Ellman’s spectrophotometric method. However, no significant inhibitory activity was observed.