Title:Protective Mechanism of Lignans from Phyllanthus amarus Against Galactosamine/ Lipopolysaccharide-Induced Hepatitis: An In-Vivo and In-Silico Studies
VOLUME: 14 ISSUE: 8
Author(s):Dnyaneshwar U. Bawankule, Priyanka Trivedi, Anirban Pal, Karuna Shanker, Manju Singh, Pooja Sharma, Feroz Khan, Anil K. Maurya, Ram K. Verma and Madan M.Gupta
Affiliation:In-Vivo Testing Laboratory, Molecular Bioprospection Department, CSIR–Central Institute of Medicinal and Aromatic Plants (CSIR–CIMAP), Lucknow 226 015, India.
Keywords:Hepatoprotective, inflammation, lignans, mice, molecular docking, Phyllanthus amarus.
Abstract:Phyllanthus amarus is a medicinal herb used in traditional Indian medicine for liver disorders. Several researches
also show that it acts primarily in the liver, but the molecules were unidentified for liver protective activity. This study was to
determine whether the lignans isolated from P. amarus attenuates the D-galactosamine (GalN) / Lipopolysaccharide (LPS)-
induced acute hepatitis in mice. Standardize mixture of lignans (slPA) isolated from leaves of P. amarus using automated
chromatographic technique was used for experiments. Experimental mice were orally pre-treated with slPA (10, 30
and100mg/kg) for 7 days before intra-peritoneal injection of GalN/LPS. Acute hepatitis in mice was confirmed by significant
increase of pro-inflammatory cytokines, and hepatotoxic markers. Pre-treatment of slPA exhibit significant liver protection in
dose dependant mannaer. In-silico molecular docking studies also suggests that lignans are preferentially more active due to
strong binding affinity against pro-inflammatory cytokines; IL-1β, IL-6, and TNF-α. The electronic parameters of lignans for
bioavailability, drug likeness and toxicity were within the acceptable limit. In-vivo and in-silico results suggest that pretreatment
of slPA exhibit potent hepatoprotection against GalN/LPS-induced hepatitis in mice and the liver protective effects
may be due to the inhibition of inflammatory mediators.
