A microdilution protocol was developed and automated using a liquid handling station, allowing the
determination of minimum inhibitory concentrations (MIC) of hydrophobic raw materials commonly used in the perfume
industry (essential oils and synthetic chemicals). Tests were performed in 96-well microtiter plates against standard
bacterial test strains and skin isolates involved in underarm malodor.
The comparison with data previously reported in the literature indicated that the protocol was suitable, yielding MIC
values that were in general agreement with those derived from manual test methods.
For the majority of active test compounds, results showed a pronounced difference in susceptibility pattern between the
Gram-positive and Gram-negative test strains used in this study. For a group of acyclic aliphatic aldehydes, a structureactivity
relationship depending on the chain length was found.
Keywords: Antimicrobial, essential oil, fragrance, high throughput assay, microdilution, minimum inhibitory concentration,
structure-activity relationship, susceptibility.
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