Isolation and Identification of a Phenol-Degrading Yeast JP-1 with High Activity and Its Degrading Characteristics and Kinetic

Author(s): Fei Pan, Ai-Hua Xu, Qing-Fu Zeng, Xin Zhao, Hai-Liang Zhu

Journal Name: Current Environmental Engineering
Continued as Current Environmental Management

Volume 1 , Issue 1 , 2014

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A yeast strain with high activity of phenol degradation was isolated from activated sludge of a wastewater treatment plant in a coking chemical factory, and identified as Candida tropicalis according to the analysis of VITEK-32 and its 18SrDNA gene sequence. The strain designated as JP-1 was able to grow on phenol as a sole carbon and energy source and it can degrade more than 90% of 1800 mg⋅L-1 phenol within 72h. The optimum pH was 6.0. Kinetic equation of phenol degradation was obtained by regression using MATLAB software. The parameters of Haldane equation were following: µmax=0.387 l⋅hr-1, Ks=4.20 mg⋅L-1, KiKi =465.6 mg⋅L-1. Cell grew was obviously inhibition by high concentration phenol and the inhibition strengthen increased with phenol increasing.

Keywords: Biodegradation, Candida tropicalis, kinetic, Haldane equation, phenol, wastewater treatment.

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Article Details

Year: 2014
Page: [4 - 9]
Pages: 6
DOI: 10.2174/2212717801666140307002510

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