TiO2~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets

Author(s): Asya S. Levina, Marina N. Repkova, Nadezhda V. Shikina, Zinfer R. Ismagilov, Natalia V. Shatskaya, Valentina F. Zarytova

Journal Name: Drug Delivery Letters

Volume 4 , Issue 2 , 2014

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Synthetic DNA molecules of 10-23 deoxyribozymes (Dz) are known to be efficient and site-specific agents for the RNA cleavage in a catalytic manner. To provide the penetration of Dz into cells, we have proposed the new delivery system for deoxyribozymes in the form of TiO2•PL-Dz nanocomposite. These nanocomposites consist of catalytically active Dz molecules noncovalently immobilized through the polylysine linker to TiO2 nanoparticles, which provide the penetration of Dz into cells. Deoxyribozymes in the prepared TiO2•PL-Dz nanocomposites were shown to retain their ability to cleave RNA targets albeit at a slower rate but with the same site-specificity and similar efficiency as free Dz. Unlike free Dz, the proposed TiO2•PL-Dz nanocomposites penetrate into cells without auxiliary actions.

Keywords: Delivery, deoxyribozymes, immobilization, nanocomposites, TiO2 nanoparticles.

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Article Details

Year: 2014
Published on: 03 August, 2014
Page: [142 - 147]
Pages: 6
DOI: 10.2174/2210303104666140222002510
Price: $25

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