Tissue engineering has yielded several successes in early clinical trials of regenerative medicine with grafting
therapeutic cells seeded into biodegradable scaffolds. However this conventional cell delivery method has limited the
field’s progress. In recent decades, we have developed a novel cell transferring method, cell sheet technology that allows
for controlled attachment and detachment of cells via simple temperature variations of a surface-intelligent temperatureresponsive
polymer:poly (N-isopropylacrylamide). It has been widely applied to create functional tissue sheets with cells
derived from various tissues to treat a wide range of diseases. Periodontal cell sheets non-invasively harvested from temperature-
responsive culture surfaces have been successfully manufactured, resulting in communicative multilayered constructs.
Transplantation of cell sheets onto periodontal defects has improved bone and tissue regeneration in animal models
and humans and shows low immunogenicity. In this review, we summarize the recent advances of techniques in cell
sheet engineering and its application for periodontal regeneration.
Keywords: Application, cell sheet technology, co-culture, micro-printing, periodontal regeneration, tissue engineering.
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