The normal development of the male reproductive system can be divided into five phases: fetal, neonatal, childhood, puberty
and adulthood. Childhood/peripuberty has yet been relatively little studied. Chemical insults during the peripubertal phase may result in
adverse consequences that may be already visible during puberty as well as during later adult life. This occurs because endocrine disruptors
often interfere in the developmental programming. The most important is to note that children are not just little adults and should be
particularly investigated. The aim of this review is to discuss the recent literature (2000-2013) on male reproductive aspects in
prepubertal toxicity assays, focusing on experimental in vivo studies, establishing a comparative analysis between the design, endpoints,
results and consequent conclusion. The studies discussed in the present review were selected based on the period of exposure. Only
studies with post-lactational exposures were included. 33 papers were included using rats, mice, rabbits or pigs as experimental model.
There is a relative scarcity of studies investigating animals in development and thus an urgent need for further studies in order to evaluate
the possible persistent effects on fertility and other reproductive parameters at adulthood. Another point is the lack of studies with chemical
mixtures, an imminent problem in modern society. It is vital to consider the refinement of alternative methods and the experimental
designs and endpoints to improve the scientific knowledge in this area.