A protective effect of α -ketobutyrate on hippocampal neuronal cells treated with H2O2 was tested. α -
Ketobutyrate concentrations were as follows: 0.5 mM, 1 mM and 5 mM. Tests were performed at the temperature of 37
ºC. Moreover, the protective effect of α-ketobutyrate on the cytotoxicity of 100 and 40 nmol/4·103 cells treated by hydrogen
peroxide was observed. Furthermore, the role of α-ketobutyrate as an energy substrate was also proven using the cis-
[Cr(C2O4)(pm)(OH2)2]+ complex as a biosensor for the carbon dioxide uptake process. All the experiments were carried
out under in-vitro conditions.