Sphere Formation Assay is not an Effective Method for Cancer Stem Cell Derivation and Characterization from the Caco-2 Colorectal Cell Line

Author(s): Hui Wu, Haihong Zhang, Yue Hu, Qiu Xia, Chenlu Liu, Yingnan Li, Bin Yu, Tiejun Gu, Xizhen Zhang, Xianghui Yu, Wei Kong

Journal Name: Current Stem Cell Research & Therapy

Volume 9 , Issue 2 , 2014

Become EABM
Become Reviewer
Call for Editor


Although the existence of cancer stem cells (CSCs) has been demonstrated in colorectal cancer, further investigation is hindered by controversies over their surface markers. The sphere formation assay is widely used as in vitro method for derivation and characterization of CSCs based on the intrinsic self-renewal property of these cells. Isolated cancer cells that form tumorspheres are generally recognized as CSCs with self-renewal and tumorigenic capacities. In this study, colon spheres grown from Caco-2 cells in the sphere formation assay were separated from other differentiated cells and characterized. Compared with Caco-2 cells, the derived colon spheres lost several CSC properties. The colon spheres contained decreased levels of specific colorectal CSC surface markers as well as low levels of ATP-binding cassette (ABC) transporters typically overexpressed in CSCs, resulting in the near loss of their chemoresistance ability. Furthermore, cells that developed as colon spheres with strong self-renewal ability in vitro lost their tumorigenic capacity in vivo compared with Caco-2 cells, which could establish tumors in non-obese diabetic/severe-combined immunodeficient (NOD/SCID) mice. The results indicated that the Caco-2 cell derived colon spheres did not consist of colorectal CSCs. Thus, the well-accepted sphere formation assay may not be an effective method for CSC isolation and characterization from the Caco-2 colorectal cancer cell line.

Keywords: Caco-2 cell line, CSCs, self-renewal, sphere formation assay, tumorigenic capacity.

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2014
Published on: 21 January, 2014
Page: [82 - 88]
Pages: 7
DOI: 10.2174/1574888X09666131217114927
Price: $65

Article Metrics

PDF: 57