Characterization of Lin-ve CD34 and CD117 Cell Population Reveals an Increased Expression in Bone Marrow Derived Stem Cells

Author(s): Neeru Jindal, Gillipsie Minhas, Sudesh Prabhakar, Akshay Anand

Journal Name: Current Neurovascular Research

Volume 11 , Issue 1 , 2014


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Abstract:

The purpose of the study was to evaluate the expression of CD45, CD34, Sca-1 and CD117 in mouse bone marrow, Lin-ve and Lin+ve population. Bone marrow cells were isolated from C57/BL6J mouse and mononuclear population was separated from rest of the cell population. With the help of Magnetic associated cell sorter (MACS), Linve and Lin+ve cells were separated from the bone marrow. The expression of CD45, CD34, Sca1 and CD117 was evaluated in bone marrow, Lin-ve and Lin+ve population by flow cytometry. We found a significant increase in the expression of CD34 and CD117 in Lin-ve as compared to the bone marrow and Lin+ve population. These findings suggest that Lin-ve population has higher expression of stem cell progenitor markers and could be useful for tissue repair and regeneration.

Keywords: Bone marrow, CD34, CD45, CD117, EPCs, Sca1, Stem cell.

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Article Details

VOLUME: 11
ISSUE: 1
Year: 2014
Published on: 08 December, 2013
Page: [68 - 74]
Pages: 7
DOI: 10.2174/1567202610666131209110035

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