Analgesic Effects of Huwentoxin-IV on Animal Models of Inflammatory and Neuropathic Pain

Author(s): Yu Liu, Zhe Wu, Dongfang Tang, Xiaohong Xun, Lichao Liu, Xianlei Li, Dongsong Nie, Yang Xiang, Jianming Yi, Jizu Yi

Journal Name: Protein & Peptide Letters

Volume 21 , Issue 2 , 2014

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Huwentoxin-IV (HWTX-IV), a peptide with 35 amino acid residues, was discovered in the venom of spider Ornithoctonus huwena. The peptide had an inhibitory effect on a tetrodotoxin-sensitive (TTX-S) sodium channel with highly sensitive to Nav1.7, an attractive target for pain release therapy. In this study we further demonstrated the analgesic effects of HWTX-IV using mouse and rat as an inflammatory pain model and/or a neuropathic pain models. In the both cases, the analgesic effects of the peptide were dose-dependent, and statistically significant. In the inflammatory model, 100 µg/kg of HWTX-IV produced an efficient reversal of hyperalgesia up to 63.6% after injection of formalin in rats with the efficiency equivalent to that of morphine at 50 µg/kg, and 200 µg/kg of HWTX-IV produced protective effect up to 55.6% after injection of acetic acid with the efficiency equivalent to that of morphine at 100 µg/kg. In the spinal nerve model, the peptide produced the longer and higher reversal effect on allodynia than Mexiletine. These results demonstrated that HWTX-IV released efficiently the acute inflammatory pain and chronic neuropathic pain in these animals, suggesting that HWTX-IV was a potential and efficient candidate for further clinical drug development against inflammatory and neuropathic pain.

Keywords: Analgesic, huwentoxin-IV, inflammatory pain, neurotoxin, neuropathic pain.

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Article Details

Year: 2014
Published on: 05 November, 2013
Page: [153 - 158]
Pages: 6
DOI: 10.2174/09298665113206660119
Price: $65

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