Advances in Protein and Peptide Sciences

Advances in Protein and Peptide Sciences

Indexed in: EBSCO, Ulrich's Periodicals Directory

Advances in Protein and Peptide Sciences is a book series focused on leading-edge research on the structure, physical properties, and functions of proteins and peptides. The series presents highly ...
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The Allosteric Properties of Hemoglobin: Insights from Natural and Site Directed Mutants

Pp. 717-791 (75)

Andrea Bellelli, Maurizio Brunori, Adriana E. Miele, Gianna Panetta and Beatrice Vallone


After over a century of extensive research, hemoglobin has become the prototype of allosteric and cooperative proteins. Its molecular structure, known in great detail, has allowed the design of hundreds of site directed mutations, aimed at interfering with its function, and thus at testing our hypotheses on the molecular mechanisms of allostery. The wealth of information thus obtained is difficult to read except for specialists, not only because it makes use of many different technical approaches, but also because of its intrinsically patchy nature. Moreover, several researchers have tried to assign specific roles to segments of the polypeptide chains, rather than to single residues, and have tested their hypotheses by multiple point mutations or by complete replacement with the homologous segment from a different hemoglobin to produce chimeric macromolecules. This approach is in great need of a revision since putative functionally relevant segments partially overlap. This review briefly describes the structure and function of hemoglobin, and analyzes the effect of point mutations, multiple mutations and segment replacement, with special attention to possible biotechnological applications, ranging from pharmacology (Hb solutions as resuscitating fluids and sources of the protein found in hemoglobinopathies for biochemical studies) to bioreactors. Occasional reference is made to site directed mutants of myoglobin, whenever this helps clarifying perplexing results obtained on hemoglobin.


Human hemoglobin, naturally occurring mutants, site directed mutants, cooperativity, allosteric core.


Dipartimento di Scienze Biochemiche “A. Rossi Fanelli”, Università degli Studi di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome; Italy.