A series of twelve compounds (Compounds RNH1–RNH12) of acid hydrazones of pyridine-3-carbohydrazide
or nicotinic acid hydrazide was synthesized and evaluated for anticonvulsant activity by MES, scPTZ, minimal clonic
seizure and corneal kindling seizure test. Neurotoxicity was also determined for these compounds by rotarod test. Results
showed that halogen substitution at meta and para position of phenyl ring exhibited better protection than ortho
substitution. Compounds RNH4 and RNH12, were found to be the active analogs displaying 6Hz ED50 of 75.4 and 14.77
mg/kg while the corresponding MES ED50 values were 113.4 and 29.3 mg/kg respectively. In addition, compound RNH12
also showed scPTZ ED50 of 54.2 mg/kg. In the series, compound RNH12 with trifluoromethoxy substituted phenyl ring
was the most potent analog exhibiting protection in all four animal models of epilepsy. Molecular docking study has also
shown significant binding interactions of these two compounds with 1OHV, 2A1H and 1PBQ receptors. Thus, N-[(meta
or para halogen substituted) benzylidene] pyridine-3-carbohydrazides could be used as lead compounds in anticonvulsant
drug design and discovery.
Keywords: Anticonvulsant, 2A1H, corneal kindling, 6Hz, MES, 1OHV, 1PBQ, scPTZ.
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