In Vitro Analysis of Proliferating CD4+ T Cells in Patients with Rheumatoid Arthritis as a Possible Predictable Marker of TNF-alpha Blockers

Author(s): Syuichi Koarada, Yuri Sadanaga, Natsumi Nagao, Satoko Tashiro, Rie Suematsu, Akihide Ohta, Yoshifumi Tada

Journal Name: Clinical Anti-Inflammatory & Anti-Allergy Drugs (Discontinued)

Volume 1 , Issue 1 , 2014


Objectives. To analyze the relationship between in vitro cytokine production in proliferating CD4+ T cells and the effect of anti-TNF-alpha (tumor necrosis factor-alpha) agents (TNF-alpha blockers) in patients with rheumatoid arthritis (RA). Methods. Peripheral blood mononuclear cells (PBMCs) from RA patients (n=19) were labeled with CFSE [5 (and 6) carboxyfluorescein diacetate, succinimidyl ester] and cultured with ConA. CD4+ T cells were assessed for production of IFN-gamma, IL-4 and TNF-alpha, and proliferation by flow cytometry. We examined association of these parameters with clinical response of TNF-alpha blockers in short, medium and long term studies. Results: Clinically good response of TNF-alpha blockers was associated with lower TNF-alpha production in proliferating CD4+ T cells in short term study (p<0.05). Increased production of IFN-gamma (p<0.05) in proliferating CD4+ T cells was associated with good response in long term study. Conclusions: In vitro evaluation of cytokine production in proliferating CD4+ T cells, prior to treatment of TNF-alpha blockers, may provide possible information of efficacy of TNF-alpha blockers in RA patients.

Keywords: Anti-inflammatory drugs, CD4+ T cells, rheumatoid arthritis, IFN-gamma, TNF-alpha, IL-4, Th1, Th2, TNFalpha blocker, proliferation, APC).

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Article Details

Year: 2014
Published on: 27 March, 2014
Page: [32 - 38]
Pages: 7
DOI: 10.2174/22127038114016660004

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