The methodological aspects are here presented for the NAPPA (Nucleic Acid Programmable Protein Arrays)
characterization by atomic force microscopy and anodic porous alumina. Anodic Porous Alumina represents also an advanced
on chip laboratory for gene expression contained in an engineered plasmid vector. The results obtained with
CdK2, CDKN1A, p53 and Jun test genes expressed on NAPPA and the future developments are discussed in terms of our
pertinent and recent Patents and of their possibility to overcome some limitations of present fluorescence detection in
probing protein-protein interaction in both basic sciences and clinical studies.