Advances in Chemical Synthesis of Structurally Modified Bioactive RNAs

Author(s): Ziyuan Li, Haipin Zhou, Xiaoming Wu, Hequan Yao

Journal Name: Current Medicinal Chemistry

Volume 20 , Issue 29 , 2013

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Methods for the chemical synthesis of RNA have been available for almost half century, and presently, RNA could be chemically synthesized by automated synthesizers, using protected ribonucleosides preactivated as phosphoramidites, which has already been covered by many reviews. In addition to advancement on synthetic methods, a variety of modifications have also been made on the synthesized oligonucleotides, and previous reviews on the general synthesis of RNAs have not covered this area. In this tutorial review, three types of modifications have been summarized standing at the viewpoint of medicinal chemistry: (1) modifications on nucleobase, comprising substituent introduction and replacement with pseudobase; (2) modifications on ribose, consisting of modifications on the 2’, 3’ or 5’-position, alternation of configuration, and conformational restriction on ribose; (3) modifications on internucleoside linkages, including amide, formacetal, sulfide, sulfone, ether, phosphorothiolate and phosphorothioate linkages. Synthetic methods achieving these modifications along with the functions or values of these modifications have also been discussed and commented on.

Keywords: Bioactive RNAs, small non-coding RNA, oligoribonucleotide, structural modification, chemical synthesis, nucleobase, pseudobase, ribose, conformational restriction, internucleoside linkage.

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Article Details

Year: 2013
Published on: 31 July, 2013
Page: [3641 - 3654]
Pages: 14
DOI: 10.2174/0929867311320290010
Price: $65

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