Expression and Characterisation of Recombinant Molecules in Transgenic Soybean

Author(s): Nicolau B. da Cunha, Andre M. Murad, Giovanni R. Vianna, Cintia Coelho, Elibio L. Rech

Journal Name: Current Pharmaceutical Design

Volume 19 , Issue 31 , 2013

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Seeds are organs specialised in accumulating proteins, and they may provide a potential economically viable platform for the large-scale production and storage of many molecules for pharmaceutical and other productive sectors. Soybean [Glycine max (L.) Merrill] has a high seed protein content and represents an excellent source of abundant and cheap biomass. Under greenhouse conditions and a daily photoperiod of 23 h of light, the soybean plant’s vegetative growth can be significantly extended by inducing more than a tenfold increase in seed production when compared with plants cultivated under field conditions. Some factors involved in the production of different recombinant proteins in soybean seeds are discussed in this review. These include transgenic system, regulatory sequences and the use of Mass Spectrometry as a new tool for molecular characterisation of seed produced recombinant proteins. The important intrinsic characteristics and possibility of genetically engineering soybean seeds, using current advances in recombinant DNA technology including metabolic engineering and synthetic biology, should form the foundation for large-scale and more precise genome modification, making this crop an important candidate as bioreactor for production of recombinant molecules.

Keywords: Molecular farming, Transgenic soybean, Glycine max, Plant seed storage proteins, Protein storage vacuoles.

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Article Details

Year: 2013
Page: [5553 - 5563]
Pages: 11
DOI: 10.2174/1381612811319310010
Price: $65

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PDF: 17