The lipid lowering effects of Senna didymobotrya (Leguminoceae) root extract (250 mg/kg) and pure isolates
(100 mg/kg) were investigated for hypolipidemic activity on Triton-induced hyperlipidemic rats (in-vivo). The extracts
and isolates showed significant (p≤0.01) reduction in serum lipid contents, and reactivation of the lipolytic enzymes,
relative to the Triton-treated group. Ethyl acetate extract exhibited the highest lipid lowering (p≤0.01) compared to other
fractions. Seven anthraquinones, obtusifolin (1), 1,6-di-O-methylemodin (2), nataloemodin-8-methyl ether (3),
chrysophanol (4), physcion (5), chrysophanol-10,10'-bianthrone (6), physcion-10,10-bianthrone (7) and stigmasterol (8)
were isolated from the ethyl acetate extract. Compounds 3, 5 and 7 displayed significant (p≤0.01) lipid lowering effects.
The hypolipidaemic activities prompted the determination of inhibition of differentiation of 3T3-L1 preadipocytes into
adipocytes by the compounds 3, 5 and 7. Only compound 7 exhibited potent anti-adipogenic activity without significant
cytotoxicity. The result indicated that extracts of S. didymobotrya root extract can provide good antidyslipidaemic and
adipocytes maturation inhibitors.