Methods for Oncogene Analysis in Thyroid FNAC
Pp. 41-63 (23)
Several oncogenes are involved in the development of thyroid cancer. In the
last 10 years, different techniques have been applied to study point mutations and
genetic rearrangements. Some of them have been used only on thyroid specimens and
need to be tested on FNAC material. Generally, these modifications can be studied by
various PCR-based techniques, and results depend on the quality and quantity of the
starting material. By carefully selecting the appropriate methods, researchers have
demonstrated that the detection of genetic alterations is feasible in a FNAC samples.
This may refine the diagnosis of thyroid cancer, especially for those samples which are
deemed cytologically inadequate.
Mutation detection, PCR, nested PCR, real time PCR, Southern blot,
Section of Endocrinology & Metabolism, Department of Internal Medicine, Endocrinology & Metabolism and Biochemistry, University of Siena, Siena, Italy.