Synthesis and Comparative Physical-chemical Characterisation of Neutral and Cationic Amphiphiles Using RP-HPLC

Author(s): Jennifer S. Squire, Luke C. Henderson, Xavier A. Conlan

Journal Name: Current Analytical Chemistry

Volume 9 , Issue 4 , 2013


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Abstract:

A series of norbornane containing amphiphiles was synthesized, their lipophilicity corresponding to neutral and cationic forms was then investigated using reverse phase HPLC (High Performance Liquid Chromatography). This series of amphiphiles incorporated varied lipophilic chain length and also varied distances between the polar/cationic head group from the norbornane scaffold. Our investigation included studying the impact of the stationary phase as a replication of a membrane for both cationic and neutral amphiphiles. The choice of stationary phase was shown to be a very important consideration for this type of measurement. In this connection, C18, Cyano and Polar columns were all investigated, the cyano column was observed to be the optimal stationary phase for the comparison of both charged and neutral amphiphiles.

Keywords: Amphiphiles, cationic lipid, capacity factor, lipophilicity, high performance liquid chromatography.

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Article Details

VOLUME: 9
ISSUE: 4
Year: 2013
Page: [653 - 658]
Pages: 6
DOI: 10.2174/1573411011309040016
Price: $65

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