Polyols or sugar alcohols are sugar-derived metabolites. The origin, metabolic fate and functions of most of them are not clear.
The monitoring of polyols can be helpful in the diagnosis and treatment of diseases, especially those of unknown etiology. The main aim
of the study was to develop and demonstrate the efficiency, reliability, sensitivity of the gas chromatography - electron capture detection
method for the quantification of polyols: D-arabitol, ribitol and erythritol in urine. In the case of polyols there is a problem with the
preparation of samples for analysis. Enrichment and separation of analytes and extract purification are difficult because of their relatively
high polarity. That is why we have developed the method of sample preparation based on solid phase extraction with using non-porous
graphitized carbon. The elaborated method was applied in autistic children in relation to the pentose phosphate pathway metabolism.
Keywords: Electron capture detection, Gas chromatography, Graphitized carbon black, Polyols, Solid phase extraction, Urine.
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Published on: 21 June, 2013
Page: [2359 - 2364]