Abstract
Polyols or sugar alcohols are sugar-derived metabolites. The origin, metabolic fate and functions of most of them are not clear. The monitoring of polyols can be helpful in the diagnosis and treatment of diseases, especially those of unknown etiology. The main aim of the study was to develop and demonstrate the efficiency, reliability, sensitivity of the gas chromatography - electron capture detection method for the quantification of polyols: D-arabitol, ribitol and erythritol in urine. In the case of polyols there is a problem with the preparation of samples for analysis. Enrichment and separation of analytes and extract purification are difficult because of their relatively high polarity. That is why we have developed the method of sample preparation based on solid phase extraction with using non-porous graphitized carbon. The elaborated method was applied in autistic children in relation to the pentose phosphate pathway metabolism.
Keywords: Electron capture detection, Gas chromatography, Graphitized carbon black, Polyols, Solid phase extraction, Urine.
Current Organic Chemistry
Title:Gas Chromatography-electron Capture Detection Method for Determination of Polyols in the Urine
Volume: 17 Issue: 20
Author(s): Wioletta Grys, Joanna Kaluzna-Czaplinska and Jacek Rynkowski
Affiliation:
Keywords: Electron capture detection, Gas chromatography, Graphitized carbon black, Polyols, Solid phase extraction, Urine.
Abstract: Polyols or sugar alcohols are sugar-derived metabolites. The origin, metabolic fate and functions of most of them are not clear. The monitoring of polyols can be helpful in the diagnosis and treatment of diseases, especially those of unknown etiology. The main aim of the study was to develop and demonstrate the efficiency, reliability, sensitivity of the gas chromatography - electron capture detection method for the quantification of polyols: D-arabitol, ribitol and erythritol in urine. In the case of polyols there is a problem with the preparation of samples for analysis. Enrichment and separation of analytes and extract purification are difficult because of their relatively high polarity. That is why we have developed the method of sample preparation based on solid phase extraction with using non-porous graphitized carbon. The elaborated method was applied in autistic children in relation to the pentose phosphate pathway metabolism.
Export Options
About this article
Cite this article as:
Grys Wioletta, Kaluzna-Czaplinska Joanna and Rynkowski Jacek, Gas Chromatography-electron Capture Detection Method for Determination of Polyols in the Urine, Current Organic Chemistry 2013; 17 (20) . https://dx.doi.org/10.2174/13852728113179990031
DOI https://dx.doi.org/10.2174/13852728113179990031 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
Call for Papers in Thematic Issues
Advances of Heterocyclic Chemistry with Pesticide Activity
Global food safety and security will continue to be a global concern for the next 50 years and beyond. Plant diseases have had a significant impact on food safety and security throughout the entire food chain, from primary production to consumption. While conventional chemical pesticides have been traditionally used for ...read more
Calculation design of covalent/metal organic framework based catalysts
This research area combines theoretical computation and screening with machine learning for the design of covalent/metal organic framework-based catalysts, bridging the disciplines of organic chemistry, physical chemistry, computational chemistry, materials science, and machine learning. It covers several critical aspects: designing and synthesizing organic catalysts for improved performance, applying computational methods ...read more
Carbohydrates conversion in biofuels and bioproducts
Biomass pretreatment, hydrolysis, and saccharification of carbohydrates, and sugars bioconversion in biofuels and bioproducts within a biorefinery framework. Carbohydrates derived from woody biomass, agricultural wastes, algae, sewage sludge, or any other lignocellulosic feedstock are included in this issue. Simulation, techno-economic analysis, and life cycle analysis of a biorefinery process are ...read more
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Pathogenesis of Type 1 Diabetes: Regulation of Adhesion Molecules and Immune Cell Trafficking
Current Immunology Reviews (Discontinued) Targeting Neurotrophic Signal Transduction Pathways in the Treatment of Mood Disorders
Current Signal Transduction Therapy Pitfalls and Solutions for the Validation of Novel Drugs in Animal Models of Disease
Current Immunology Reviews (Discontinued) An Update of the Review of Neuropsychological Consequences of HIV and Substance Abuse: A Literature Review and Implications for Treatment and Future Research
Current Drug Abuse Reviews Gene Expression Suggests Spontaneously Hypertensive Rats May Have Altered Metabolism and Reduced Hypoxic Tolerance
Current Neurovascular Research Emerging Therapies in Relapsing-Remitting Multiple Sclerosis
Reviews on Recent Clinical Trials Biologic Agents for Treatment of Rheumatoid Arthritis
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Anti-Angiogenic Approaches to Malignant Gliomas
Current Cancer Drug Targets Promising Pharmacological, Molecular and Cellular Treatments of Autoimmune Hepatitis
Current Pharmaceutical Design Current Options in the Treatment of Mitochondrial Diseases
Recent Patents on CNS Drug Discovery (Discontinued) Current Treatment Strategies for Multiple Sclerosis - Efficacy Versus Neurological Adverse Effects
Current Pharmaceutical Design Role of microRNAs on Blood Brain Barrier Dysfunction in Vascular Cognitive Impairment
Current Drug Delivery Review of Endocrine Manifestations of Antiphospholid Syndrome
Current Rheumatology Reviews Nuclear Magnetic Resonance Spectroscopy and Genetic Disorders
Current Medicinal Chemistry The HLA Region and Autoimmune Disease: Associations and Mechanisms of Action
Current Genomics Substance Abuse and Movement Disorders
Current Drug Abuse Reviews Monoclonal Antibodies in Rare Acquired Thrombotic Disorders: The Paradigms of Paroxysmal Nocturnal Hemoglobinuria, Thrombotic Thrombocytopenic Purpura and Central Retinal Vein Occlusion
Current Vascular Pharmacology The Beneficial Effects of Sulfur-containing Amino Acids on Cisplatininduced Cardiotoxicity and Neurotoxicity in Rodents
Current Medicinal Chemistry Chemotherapy and Delivery in the Treatment of Primary Brain Tumors
Current Clinical Pharmacology Endotherapia
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry