Novel membranes were fabricated from fungal chitosan (Cs) and starch based super absorbent polymer (SAP)
for hemostatic application. The commercial production of Cs through alkaline deacetylation of crustacean chitin includes
many drawbacks. Fungal Cs production, by a more eco-compatible technique, has become an alternative source for the
traditional one. In this study, the production of fungal Cs was executed in a bioreactor from the mycelia of Absidia coerulea.
The maximum obtained fungal Cs was 0.55 g/L after 48 h. The fabrication of Cs-SAP membranes was approached
subsequently using two methods, physical blend of two polymers, and Cs-SAP sub-layer. To evaluate the homeostatic effect
of Cs – SAP membranes on blood, erythrocyte sedimentation test was conducted in vitro. Since increasing the Cs
concentration from 0.5 to 2 % w/v in the fabricated Cs – SAP membranes, reduces the erythrocyte sedimentation time
from 69.8 to 62.3 min, respectively, while increasing the concentration of SAP (0.12-0.5 % w/v) has less or no significant
effect on erythrocyte sedimentation time. Sub-layered 2L8 membrane significantly reduced ESR (P< 0.05) by 22%, while
physically blended 11B8 membrane diminished ESR by only 12% compared to the control. Furthermore, these membranes
were investigated by FT-IR, SEM, tensile, antimicrobial activity and cytotoxicity. Chitosan-SAP membranes can
be described as bio-membranes with a homogeneous matrix, stable structure and interesting mechanical properties, with
great possibilities of utilization in hemostasis.