An estrogen selected molecular imprinted polymer (MIP) has been developed to be used as an alternative sorbent
in solid phase extraction of serum samples before capillary electrophoresis analysis. Following a heat polymerization,
MIP has been synthesized using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA)
as crosslinker, benzoyl peroxide as radical initiator and 17β- estradiol as template, and toluene as porogen. The optimized
molecular imprinted solid phase extraction (MISPE) was compared to traditional C18 solid phase extraction to purify
estrogens from complex matrices. It was concluded that the MISPE system developed is useful for the selective extraction
of 17β- estradiol and its major natural related estrogens, estriol and estrone, from serum samples with minimal interferences
and high recoveries.