A peptide microarray fabricated on a non-fouling phosphatidylcholine-polymer-coated surface for a high-fidelity analysis of a cellular kinome

Author(s): H. Ikeda, J. Kamimoto, T. Yamamoto, A. Hata, Y. Otsubo, T. Niidome, M. Fukushima, T. Mori, Y. Katayama

Journal Name: Current Medicinal Chemistry

Volume 20 , Issue 35 , 2013

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Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.

Keywords: Kinome peptide, microarray, non-fouling, phosphatidylcholine, protein kinase.

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Article Details

Year: 2013
Page: [4419 - 4425]
Pages: 7
DOI: 10.2174/09298673113209990142
Price: $65

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