This study investigated the effects of the L-17 compound of the group of substituted 5R1, 6H2-
1,3,4-thiadiazine-2-amines on the immune response and the plasma level of circulating cytokines in acute
myocardial infarction (MI) in rats.
The study was based upon experimental work which demonstrated the role of local and systemic inflammatory reactions
in MI. Acute MI in rats was induced by left coronary artery coagulation. Histological study of the myocardium sections
has been carried out at the 1th and 7th days of the experimental myocardial infarction. Serum activity of creatine phosphokinase
(CPK), aspartate aminotransferase (AST), isoenzymes 1 and 2 and lactate dehydroge nase (LDH1-2) were investigated
at days 1stand 7th. ELISA analysis for plasma cytokine levels was performed using commercially available test
kits following the manufacturer's instructions.
Biochemical analysis in animals with the administration of the L-17 compound after MI showed that the AST and CPK
levels at days 5 and 7 of experiments did not differ significantly from the values of intact animals.
In animals of the group with MI without the administration of the L-17 compound, the IL-1 level 8 times and the TNF
level 7.8 times exceeded the normal indicators, while the use of L-17 compound in the therapy resulted in only 1.8 times
increase of IL-1 level and 4.7 times increase of TNF level in comparison with the norm.
Thus, the introduction of L-17 compound in case of experimental MI delays exudative/alternative phase of inflammation,
accelerates granulocytic and decreased the inflammation and anti-inflammation interleukins level.