Abstract
The results accrued in the last few years have clearly showed that recombinant antibodies, and specifically single- domain antibodies, represent valid alternatives to conventional IgGs for in vivo imaging. It does not simply mean that antibody fragments can substitute full-length antibodies, but that they are substantially more suitable for some applications and can perform other functions for which no real alternative is available. Brain imaging with multi-functional probes is an evident example, but the promising results obtained with micro-PET and –SPECT in murine models could lead in short time to a revolutionary change in clinical diagnostics. Brilliant applications of single-domain antibody-dependent imaging have enabled us to understand how the tracer mass and avidity can be engineered to modulate pharmacokinetic features such as clearance, tumor penetration, and binding affinity with the aim of optimizing specific responses. The potential of these reagents and the increasing interest for them is evidenced by the exponential growth of publications and the multiplication of the proposed applications in which they are used. This review wishes to provide an update of this fast moving subject and to indicate what may be the next foreseeable technical progress.
Keywords: Antibody functionalization, fresh tumor cells, imaging, nanobodies, PET/SPECT, phage display panning, singledomain antibodies, tumor clonal variability.
Current Medicinal Chemistry
Title:Perspectives Offered by Single-Domain Antibodies in Clinical Diagnostic of Pediatric Tumors
Volume: 20 Issue: 17
Author(s): A. de Marco
Affiliation:
Keywords: Antibody functionalization, fresh tumor cells, imaging, nanobodies, PET/SPECT, phage display panning, singledomain antibodies, tumor clonal variability.
Abstract: The results accrued in the last few years have clearly showed that recombinant antibodies, and specifically single- domain antibodies, represent valid alternatives to conventional IgGs for in vivo imaging. It does not simply mean that antibody fragments can substitute full-length antibodies, but that they are substantially more suitable for some applications and can perform other functions for which no real alternative is available. Brain imaging with multi-functional probes is an evident example, but the promising results obtained with micro-PET and –SPECT in murine models could lead in short time to a revolutionary change in clinical diagnostics. Brilliant applications of single-domain antibody-dependent imaging have enabled us to understand how the tracer mass and avidity can be engineered to modulate pharmacokinetic features such as clearance, tumor penetration, and binding affinity with the aim of optimizing specific responses. The potential of these reagents and the increasing interest for them is evidenced by the exponential growth of publications and the multiplication of the proposed applications in which they are used. This review wishes to provide an update of this fast moving subject and to indicate what may be the next foreseeable technical progress.
Export Options
About this article
Cite this article as:
de Marco A., Perspectives Offered by Single-Domain Antibodies in Clinical Diagnostic of Pediatric Tumors, Current Medicinal Chemistry 2013; 20 (17) . https://dx.doi.org/10.2174/0929867311320170004
DOI https://dx.doi.org/10.2174/0929867311320170004 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
Call for Papers in Thematic Issues
Advances in Medicinal Chemistry: From Cancer to Chronic Diseases.
The broad spectrum of the issue will provide a comprehensive overview of emerging trends, novel therapeutic interventions, and translational insights that impact modern medicine. The primary focus will be diseases of global concern, including cancer, chronic pain, metabolic disorders, and autoimmune conditions, providing a broad overview of the advancements in ...read more
Approaches to the treatment of chronic inflammation
Chronic inflammation is a hallmark of numerous diseases, significantly impacting global health. Although chronic inflammation is a hot topic, not much has been written about approaches to its treatment. This thematic issue aims to showcase the latest advancements in chronic inflammation treatment and foster discussion on future directions in this ...read more
Cellular and Molecular Mechanisms of Non-Infectious Inflammatory Diseases: Focus on Clinical Implications
The Special Issue covers the results of the studies on cellular and molecular mechanisms of non-infectious inflammatory diseases, in particular, autoimmune rheumatic diseases, atherosclerotic cardiovascular disease and other age-related disorders such as type II diabetes, cancer, neurodegenerative disorders, etc. Review and research articles as well as methodology papers that summarize ...read more
Chalcogen-modified nucleic acid analogues
Chalcogen-modified nucleosides, nucleotides and oligonucleotides have been of great interest to scientific research for many years. The replacement of oxygen in the nucleobase, sugar or phosphate backbone by chalcogen atoms (sulfur, selenium, tellurium) gives these biomolecules unique properties resulting from their altered physical and chemical properties. The continuing interest in ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Restoration of Chemoresistance Mechanism by Novel Drug Therapies in Breast Cancer Cell Lines
Current Drug Therapy Targeting the Endocannabinoid System for the Treatment of Cancer – A Practical View
Current Topics in Medicinal Chemistry Drug Targeting Strategies in Cancer Treatment: An Overview
Mini-Reviews in Medicinal Chemistry Interplay between Epigenetics & Cancer Metabolism
Current Pharmaceutical Design Patents in Cancer Stem Cells
Recent Patents on Biomarkers Tumor Stem Cell Niches: A New Functional Framework for the Action of Anticancer Drugs
Recent Patents on Anti-Cancer Drug Discovery The Urokinase Plasminogen Activator System: A Target for Anti-Cancer Therapy
Current Cancer Drug Targets Aberrant Regulation of Translation Initiation in Tumorigenesis
Current Molecular Medicine Promising Pharmacological, Molecular and Cellular Treatments of Autoimmune Hepatitis
Current Pharmaceutical Design Neuroimaging Features of Acquired Metabolic and Toxic Encephalopathies
Current Medical Imaging Role of the Non-Receptor Tyrosine Kinase Fes in Cancer
Current Medicinal Chemistry Bench to Bedside Targeting of FLT3 in Acute Leukemia
Current Drug Targets A New Strategy to Target Acute Myeloid Leukemia Stem and Progenitor Cells Using Chidamide, a Histone Deacetylase Inhibitor
Current Cancer Drug Targets From Amino Acids to Proteins as Targets for Metal-based Drugs
Current Drug Metabolism Clinical Presentation, Outcome and Risk Factors of Late-Onset Non- Infectious Pulmonary Complications After Allogeneic Stem Cell Transplantation
Current Stem Cell Research & Therapy HIF-1α Modulates Energy Metabolism in Cancer Cells by Inducing Over-Expression of Specific Glycolytic Isoforms
Mini-Reviews in Medicinal Chemistry (1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives
Current Drug Targets Personalized Therapies in Pediatric Inflammatory and Autoimmune Diseases
Current Pharmaceutical Design Mechanisms for Targeted Delivery of Nanoparticles in Cancer
Current Pharmaceutical Design Unveiling the Role of Nuclear Transport in Epithelial-to-Mesenchymal Transition
Current Cancer Drug Targets