Raman Spectroscopic Markers for the Xenopus Laevis Oocyte Expression System

Author(s): Noriyuki Masuda, Haim Abitan, Maria de los Angeles Tejada, Dan Klaerke, Henrik Bohr, Claus Helix-Nielsen

Journal Name: Current Physical Chemistry

Volume 3 , Issue 2 , 2013


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Abstract:

Studying membrane protein function using Xenopus laevis oocytes has been proven to be a successful tool since its introduction more than three decades ago. Due to their great availability, large size and ease of handling, X. laevis oocytes are often superior when compared to other expression systems such as Escherichia coli or eukaryote expression systems. The Xenopus laevis oocyte expression system is able to efficiently transcribe and translate injected genetic information and to assemble, process, and target protein products. Protein characterization studies in intact whole cell injected oocytes are done using established techniques such as electrophysiology, transport assays, and calcium imaging. Recently Xenopus oocytes have been analyzed using in-vivo NMR spectroscopy, and we wondered if it would be possible to use Raman spectroscopy for non-invasive analysis of single oocytes. We present here evidence that it is possible to identify Raman bands arising from heterologous expressed membrane proteins in stage VI oocytes. This opens the possibility for integrating the Raman analysis with already established protein expression methods in order to yield complementary information about membrane protein structure and function.

Keywords: Xenopus Oocyte, Raman spectroscopy, Slack channels, Protein expression.

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Article Details

VOLUME: 3
ISSUE: 2
Year: 2013
Published on: 31 March, 2013
Page: [151 - 154]
Pages: 4
DOI: 10.2174/1877946811303020005
Price: $25

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