Carbohydrates are complex and structurally diverse compounds in nature with key roles in a broad range of life processes including
signal transduction, carcinogenesis and immune responses. Many natural products contain oligosaccharides that are vital for their
biological activity. Despite ongoing challenges, tremendous progresses have been made in recent years for the synthesis of carbohydrates.
The chemical glycosylation methods have become more sophisticated and the synthesis of oligosaccharides has become more predictable.
Nonetheless, thanks to their straightforward stereoselectivity and efficiency, carbohydrate-processing enzymes follow being a powerful
practical alternative in a wide set of synthetic applications targeted to the obtainment of natural oligosaccharides, glycoconjugates
and their analogues. In this review, recurring to many practical examples, a general updated overview of the behavior, the advantages and
the drawbacks related to the application of glycosyltransferases, glycosylhydrolases and glycosynthases in the oligosaccharide synthesis
will be provided.