The application of specific chemical strategies to alter the structure of enzymes in order to improve their catalytic properties in
different biotransformations has been a central role in biocatalysis in recent years. Particularly, cycloaddition reactions have represented a
successfully methodology for enzyme chemistry because it is chemoselective and extremely rate-accelerated in aqueous media. Some of
the most current examples in the use of click chemistry for site-specific modification and immobilization of enzymes has been reviewed
in the present article.
Keywords: 1, 3-dipolar cycloaddition, Diels-Alder reaction, chemical modification, biocatalysis, biotransformations, selectivity, chemoselectivity,
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