Title:Nucleic Acids Electro-transfer: From Bench to Bedside
VOLUME: 14 ISSUE: 3
Author(s):Sophie Chabot, Christelle Rosazza, Muriel Golzio, Andreas Zumbusch, Justin Teissie and Marie-Pierre Rols
Affiliation:CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), BP 64182, 205 route de Narbonne, F-31077 Toulouse, France.
Keywords:Electroporation, gene delivery, RNAi, tumor, Nucleic Acids, Electro-transfer, Antitumor Drugs, macromolecules, Nucleic Acid, DNA, LNA, SiRNA, Plasmid
Abstract:There are a number of stigmas attached to the development of antitumor drugs such as their safe and efficient delivery into
target cells or tissues. The one such case is that of macromolecules, such as nucleic acids where it poses severe limits. From this point of
view, electro-pulsation proves to be a promising method for cancer-associated gene therapy. It involves the direct application of electric
pulses on cells or tissues which leads to a transient permeabilization of their plasma membrane allowing efficient in vitro and in vivo
delivery of exogenous molecules. The present review probes the electrotransfer of nucleic acids, the nature of nucleic acids (plasmid
DNA, mRNA, siRNA, LNA...) which can be electrotransferred and the mechanism of their electrotransfer.
