Prediction of Turnover Number of Cellulose 1,4-Beta-Cellobiosidase

Author(s): Shaomin Yan, Guang Wu

Journal Name: Protein & Peptide Letters

Volume 20 , Issue 3 , 2013


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Abstract:

The turnover number is an important parameter to distinguish whether an enzyme is practically workable. Therefore the prediction of turnover number of enzyme will reduce the workload to conduct time-consuming and costly experiments to determine the turnover number. However, no studies have been so far conducted to predict them with respect to cellulose 1,4-beta-cellobiosidase, which is an enzyme used in industries, especially in bio-fuel industry. It is important to develop methods to predict the turnover numbers of cellulose 1,4-beta-cellobiosidases from both wild-type and mutations. In this study, we used neural network models with different amino acid properties, pH levels, temperatures and substrates as inputs to predict the turnover number. The results show that 11 out of 25 amino acid properties analyzed can work as predictor and the amino acid distribution probability is the best one because it can reach smaller mean squared errors during convergence and higher correlation coefficient in two-layer neural network models. This study demonstrates the probability that the neural network model can approximately predict the turnover number of cellulose 1,4-betacellobiosidase.

Keywords: Cellulose 1, 4-beta-cellobiosidase, prediction, turnover number, parameter, enzyme, experiment, bio-fuel industry, mutation, neural network model, amino acid

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Article Details

VOLUME: 20
ISSUE: 3
Year: 2013
Published on: 18 January, 2013
Page: [255 - 264]
Pages: 10
DOI: 10.2174/0929866511320030004
Price: $65

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