Electrochemical Study of DNA Damaged by Oxidation Stress

Author(s): Ondrej Zitka, Sona Krizkova, Sylvie Skalickova, Pavel Kopel, Petr Babula, Vojtech Adam, Rene Kizek

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 16 , Issue 2 , 2013

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Abstract:

Many compounds can interact with DNA leading to changes of DNA structure as point mutation and bases excision, which could trigger some metabolic failures, which leads to the changes in DNA structure resulting in cancer. Oxidation of nucleic acid bases belongs to the one of the mostly occurred type of DNA damaging leading to the above mentioned phenomena. The investigation of processes of DNA oxidation damage is topical and electrochemical methods include a versatile and sensitive tool for these purposes. 8-hydroxydeoxyguanosine (8-OHdG) is the most widely accepted marker of DNA damage. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generate several other products within the double helix besides mentioned oxidation products of nucleic acid bases. The basic electrochemical behaviour of nucleic acids bases on various types of carbon electrodes is reviewed. Further, we address our attention on description of oxidation mechanisms and on detection of the most important products of nucleic bases oxidation. The miniaturization of detector coupled with some microfluidic devices is suggested and discussed.

The main aim of this review is to report the advantages and features of the electrochemical detection of guanine oxidation product as 8-OHdG and other similarly produced molecules as markers for DNA damage.

Keywords: Miniaturization, nucleic acid bases, oxidation damage of DNA, voltammetry.

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Article Details

VOLUME: 16
ISSUE: 2
Year: 2013
Page: [130 - 141]
Pages: 12
DOI: 10.2174/1386207311316020007
Price: $65

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