Abstract
In the present study, polyethylenimine (PEI, 1800 Da) and liposome were combined in order to improve gene expression. A new gene delivery system (polycationic liposome) was developed by modification of liposomes with lipoploymers constructed from acrylate or bromoalkane derivatives. The polycationic liposome-plasmid DNA (pDNA) complexes were characterized for their size, zeta potential and ability for DNA condensation. Luciferase reporter gene was used for the determination of transfection efficiency in Neuro2A cells. While mean particle size of prepared vectors ranged from 75 to 520 nm, the zeta potential varied from 11-35 mV. Transfection activity of selected non-viral vectors was higher than that of PEI 1800 Da and PEI 25 KDa. The transfection activity of polycationic liposomes was reduced after replacement of bromoalkane derivatives by acrylates in the structure of lipopolymers. Furthermore, gene carriers described in this study showed low cytotoxicity. The results show that inclusion of hydrophobic PEI derivatives in liposome structure can improve the transfection efficiency but the lipopolymer structure determines the gene expression efficiency of vectors
Keywords: Lipopolymer, liposomes, non-viral gene delivery, polyethylenimine.
Current Nanoscience
Title:The Effect of Lipopolymer Structure on the Transfection Efficiency of Hydrophobic Polyethylenimine-based Cationic Nanoliposomes
Volume: 8 Issue: 5
Author(s): Mohammad Ramezani, Bizhan Malaekeh-Nikouei, Susan Malekzadeh, Mohammad Reza Baghayeripour and Mohammad Malaekeh-Nikouei
Affiliation:
Keywords: Lipopolymer, liposomes, non-viral gene delivery, polyethylenimine.
Abstract: In the present study, polyethylenimine (PEI, 1800 Da) and liposome were combined in order to improve gene expression. A new gene delivery system (polycationic liposome) was developed by modification of liposomes with lipoploymers constructed from acrylate or bromoalkane derivatives. The polycationic liposome-plasmid DNA (pDNA) complexes were characterized for their size, zeta potential and ability for DNA condensation. Luciferase reporter gene was used for the determination of transfection efficiency in Neuro2A cells. While mean particle size of prepared vectors ranged from 75 to 520 nm, the zeta potential varied from 11-35 mV. Transfection activity of selected non-viral vectors was higher than that of PEI 1800 Da and PEI 25 KDa. The transfection activity of polycationic liposomes was reduced after replacement of bromoalkane derivatives by acrylates in the structure of lipopolymers. Furthermore, gene carriers described in this study showed low cytotoxicity. The results show that inclusion of hydrophobic PEI derivatives in liposome structure can improve the transfection efficiency but the lipopolymer structure determines the gene expression efficiency of vectors
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Cite this article as:
Ramezani Mohammad, Malaekeh-Nikouei Bizhan, Malekzadeh Susan, Reza Baghayeripour Mohammad and Malaekeh-Nikouei Mohammad, The Effect of Lipopolymer Structure on the Transfection Efficiency of Hydrophobic Polyethylenimine-based Cationic Nanoliposomes, Current Nanoscience 2012; 8 (5) . https://dx.doi.org/10.2174/157341312802884535
DOI https://dx.doi.org/10.2174/157341312802884535 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
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