Lacticin LC14, a New Bacteriocin Produced by Lactococcus lactis BMG6.14: Isolation, Purification and Partial Characterization

Author(s): Samar Lasta, Hadda Ouzari, Nicolas Andreotti, Ziad Fajloun, Pascal Mansuelle, Abdellatif Boudabous, François Sampieri, Jean Marc Sabatier.

Journal Name: Infectious Disorders - Drug Targets
(Formerly Current Drug Targets - Infectious Disorders)

Volume 12 , Issue 4 , 2012

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Abstract:

A new bacteriocin, lacticin LC14, produced by Lactococcus lactis BMG6.14, was isolated and characterized. It was purified to homogeneity from overnight broth culture by ammonium sulfate precipitation, Sep-Pak chromatography, and two steps of reversed-phase HPLC. Lacticin LC14 showed bactericidal-type antimicrobial activity against several lactic acid bacteria and pathogenic strains including Listeria monocytogenes. It was inactivated by proteinase K and pronase E, but was resistant to papain, lysozyme, lipase and catalase. Lacticin LC14 was heat resistant, stable over a wide range of pH (2-10) and after treatment by solvents and detergents. Its N-terminal end was found unreactive towards Edman sequencing. Based on MALDI-TOF mass spectrometry, its molecular mass was 3333.7 Da. LC14 amino acid composition revealed a high proportion of hydrophobic residues, but no modified ones. LC14 may be able to challenge other well known other bacteriocins in probiotic and therapeutic applications.

Keywords: Antimicrobial activity, Bacteriocin, Lactic acid bacteria, Lacticin LC14, Listeria monocytogenes, Lactococcus lactis subsp. lactis BMG6.14, ammonium sulfate precipitation, chromatography, chromatography, pathogenic strains, lysozyme, lipase and catalase, amino acid, hydrophobic residues

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Article Details

VOLUME: 12
ISSUE: 4
Year: 2012
Page: [316 - 325]
Pages: 10
DOI: 10.2174/187152612801319276
Price: $65

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