Abstract
Imprinting of macromolecule such as proteins with molecular imprinting technique remains challenge because of their bulkiness, structural complexity and sensitivity of denaturation. The purpose of this study was to synthesize a Lys-MIP film on the surface of the MWNTs by surface molecular imprinting technique, and subsequently selectively recognized and purified Lys from egg white. The Lys-MIP film was prepared using AAm as functional monomer, MBA as cross-linker, APS and TEMED as initiator under the optimized conditions. The polymer was characterized by TEM, FT-IR, TGA, and BET, as well. The equilibrium adsorption experiments and dynamic adsorption experiments were carried out to determine the binding capacity and the equilibrium time. The results showed that adsorption capacity of the Lys-MIP film was 20.89 mg g-1 at 0.50 mg mL-1, and adsorption capacity was 15.37 mg g-1 for the NIP. 82.10% of binging was obtained within 30 min and the adsorption equilibrium was achieved within 300 min. The selectivity adsorption experiments showed the Lys-MIP film had higher affinity and capacity for Lys than that for the competitive proteins, such as Cyt C, Mb, Hb and BSA. The relative selectivity coefficients for Lys/Cyt C, Lys/Mb, Lys/Hb, and Lys/BSA were 1.30, 1.30, 3.12 and 2.82, respectively. Purification experiment of Lys from egg white showed that adsorption capacity of the Lys-MIP film was 1.86 times more than that of the NIP. All these results proved that Lys-MIP film possessed high adsorption capacity and excellent selectivity. This material has great potential for separation of Lys in real samples.
Keywords: Egg white, Lysozyme purification, Multi-wall carbon nanotubes, Molecular imprinting, Protein recognition, Surface imprinting
Current Organic Chemistry
Title:Preparation and Evaluation of Lysozyme Molecularly Imprinted Polymer Film on the Surface of Multi-wall Carbon Nanotubes
Volume: 16 Issue: 12
Author(s): Guozhen Fang, Xiaorui Zhai, Qiliang Deng, Shifang Yuan, Meirong Cao and Shuo Wang
Affiliation:
Keywords: Egg white, Lysozyme purification, Multi-wall carbon nanotubes, Molecular imprinting, Protein recognition, Surface imprinting
Abstract: Imprinting of macromolecule such as proteins with molecular imprinting technique remains challenge because of their bulkiness, structural complexity and sensitivity of denaturation. The purpose of this study was to synthesize a Lys-MIP film on the surface of the MWNTs by surface molecular imprinting technique, and subsequently selectively recognized and purified Lys from egg white. The Lys-MIP film was prepared using AAm as functional monomer, MBA as cross-linker, APS and TEMED as initiator under the optimized conditions. The polymer was characterized by TEM, FT-IR, TGA, and BET, as well. The equilibrium adsorption experiments and dynamic adsorption experiments were carried out to determine the binding capacity and the equilibrium time. The results showed that adsorption capacity of the Lys-MIP film was 20.89 mg g-1 at 0.50 mg mL-1, and adsorption capacity was 15.37 mg g-1 for the NIP. 82.10% of binging was obtained within 30 min and the adsorption equilibrium was achieved within 300 min. The selectivity adsorption experiments showed the Lys-MIP film had higher affinity and capacity for Lys than that for the competitive proteins, such as Cyt C, Mb, Hb and BSA. The relative selectivity coefficients for Lys/Cyt C, Lys/Mb, Lys/Hb, and Lys/BSA were 1.30, 1.30, 3.12 and 2.82, respectively. Purification experiment of Lys from egg white showed that adsorption capacity of the Lys-MIP film was 1.86 times more than that of the NIP. All these results proved that Lys-MIP film possessed high adsorption capacity and excellent selectivity. This material has great potential for separation of Lys in real samples.
Export Options
About this article
Cite this article as:
Fang Guozhen, Zhai Xiaorui, Deng Qiliang, Yuan Shifang, Cao Meirong and Wang Shuo, Preparation and Evaluation of Lysozyme Molecularly Imprinted Polymer Film on the Surface of Multi-wall Carbon Nanotubes, Current Organic Chemistry 2012; 16 (12) . https://dx.doi.org/10.2174/138527212800672547
DOI https://dx.doi.org/10.2174/138527212800672547 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
Call for Papers in Thematic Issues
Advances of Heterocyclic Chemistry with Pesticide Activity
Global food safety and security will continue to be a global concern for the next 50 years and beyond. Plant diseases have had a significant impact on food safety and security throughout the entire food chain, from primary production to consumption. While conventional chemical pesticides have been traditionally used for ...read more
Calculation design of covalent/metal organic framework based catalysts
This research area combines theoretical computation and screening with machine learning for the design of covalent/metal organic framework-based catalysts, bridging the disciplines of organic chemistry, physical chemistry, computational chemistry, materials science, and machine learning. It covers several critical aspects: designing and synthesizing organic catalysts for improved performance, applying computational methods ...read more
Carbohydrates conversion in biofuels and bioproducts
Biomass pretreatment, hydrolysis, and saccharification of carbohydrates, and sugars bioconversion in biofuels and bioproducts within a biorefinery framework. Carbohydrates derived from woody biomass, agricultural wastes, algae, sewage sludge, or any other lignocellulosic feedstock are included in this issue. Simulation, techno-economic analysis, and life cycle analysis of a biorefinery process are ...read more
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Natural Agents Modulating ACE-2: A Review of Compounds with Potential against SARS-CoV-2 Infections
Current Pharmaceutical Design Biological Evaluation of 3-Acyl-2-Arylamino-1,4-Naphthoquinones as Inhibitors of Hsp90 Chaperoning Function
Current Topics in Medicinal Chemistry A Survey on Machine Learning Based Medical Assistive Systems in Current Oncological Sciences
Current Medical Imaging Stereochemistry as a Determinant of the Anti-Leukemic Potency of Halopyridyl and Thiazolyl Thiourea Compounds
Letters in Drug Design & Discovery Inhibitory Effect of Fruit Juices on the Doxorubicin Metabolizing Activity of Carbonyl Reductase 1
Drug Metabolism Letters Curcumin: A Natural Product for Diabetes and its Complications
Current Topics in Medicinal Chemistry Selenoproteins and their Role in Oxidative Stress and Inflammation
Current Chemical Biology Novel Conjugated Unsaturated Ketones with Submicromolar Potencies Towards some Leukemic and Colon Cancer Cells
Medicinal Chemistry Study of the Nucleotide-Binding Domain 1 of the Human Transporter Protein MRP6
Protein & Peptide Letters Biodegradation Aspects of Carbendazim and Sulfosulfuron: Trends, Scope and Relevance
Current Medicinal Chemistry Osteoporotic Fracture Healing: Potential Use of Medicinal Plants from the Tropics
Current Drug Targets MiR-134, Mediated by IRF1, Suppresses Tumorigenesis and Progression by Targeting VEGFA and MYCN in Osteosarcoma
Anti-Cancer Agents in Medicinal Chemistry ZnS Nanoparticles: An Efficient Catalyst for the One-Pot Synthesis of Spiro [Pyrrolidine-2, 3'-Oxindole] Derivatives from Phenothiazinyl Chalcones
Letters in Organic Chemistry Anticancer Activity of Ferrocenylthiosemicarbazones
Anti-Cancer Agents in Medicinal Chemistry Neuropeptidomics: Improvements in Mass Spectrometry Imaging Analysis and Recent Advancements
Current Protein & Peptide Science Potential for Predicting Toxicity and Response of Fluoropyrimidines in Patients
Current Drug Targets Fluorescent Cisplatin Analogues and Cytotoxic Activity
Current Medicinal Chemistry Neuroprotective Effects of Ellagic Acid in Alzheimer's Disease: Focus on Underlying Molecular Mechanisms of Therapeutic Potential
Current Pharmaceutical Design Non-Ceruloplasmin Copper as a Stratification Biomarker of Alzheimer’s Disease Patients: How to Measure and Use It
Current Alzheimer Research Recent Progress in Synthesis, Vibrational Characterization and Applications Trend of Conjugated Polymers/Carbon Nanotubes Composites
Current Organic Chemistry