Abstract
Hypericum perforatum is a medicinal herb possessing ability for protecting neurons from oxidative stress. Since nitric oxide (NO) may be protective against oxidative stress-induced cell death as occurs in glucose deprivation (GD)-induced neurotoxicity, whether a standardized extract of H. perforatum (HP) increases the NO-mediated neuroprotective effect in GD-PC12 cells was investigated. Induced death in PC12 cells by GD exposure for 18 h was partially prevented by cell incubation with sodium nitroprusside (SNP), a NO-donor. SNP increased survival and nitrite production in GD-cells in a concentration-dependent manner. Co-incubation of cells with 10 μM SNP plus 50-100 μg/ml HP under GD insult significantly prevented GD-induced cell death to a higher extent than SNP alone as shown by an augmentation of cell survival and intracellular bcl-2 levels and a decrease of lipid peroxidation, caspase-3 activation and PARP cleavage. Cytoprotection by the NO-donor was almost abolished by the use of a NO scavenger and potentiated by the presence of superoxide dismutase. SNP and/or HP neuroprotection on GD-cells was significantly reversed by rotenone treatment. These results suggest that: (1) SNP could protect PC12 cells from GD-induced cytotoxicity through NO generation and (2) the enhancement of the SNP-mediated neuroprotective effect on GD-cells by HP might arise in part through scavenging of reactive oxygen species (ROS) and inhibition of mitochondrial dysfunction associated with the hypoglycemic episode. This current finding might highlight the development of therapeutic strategies aimed at manipulating NO-donors in combination with herb supplements containing ROS scavenger compounds for prophylaxis from brain ischemia.
Keywords: Glucose depriviation, Hypericum Perforatum, Brain Ischemia, Neuroprotection, Nitric oxide, reactive oxygen species, Pheochromocytoma, DMEM, PTIO, DCFA-DA, Digiscan Microplate Reader, Caspase-3 Activity assay
CNS & Neurological Disorders - Drug Targets
Title:SNP-Mediated Neuroprotection under Glucose Deprivation Is Enhanced by Hypericum perforatum
Volume: 11 Issue: 2
Author(s): Maria Munoz, Paloma Bermejo-Bescos, Carmen Romero, Juana Benedi, Sagrario Martin-Aragon
Affiliation:
Keywords: Glucose depriviation, Hypericum Perforatum, Brain Ischemia, Neuroprotection, Nitric oxide, reactive oxygen species, Pheochromocytoma, DMEM, PTIO, DCFA-DA, Digiscan Microplate Reader, Caspase-3 Activity assay
Abstract: Hypericum perforatum is a medicinal herb possessing ability for protecting neurons from oxidative stress. Since nitric oxide (NO) may be protective against oxidative stress-induced cell death as occurs in glucose deprivation (GD)-induced neurotoxicity, whether a standardized extract of H. perforatum (HP) increases the NO-mediated neuroprotective effect in GD-PC12 cells was investigated. Induced death in PC12 cells by GD exposure for 18 h was partially prevented by cell incubation with sodium nitroprusside (SNP), a NO-donor. SNP increased survival and nitrite production in GD-cells in a concentration-dependent manner. Co-incubation of cells with 10 μM SNP plus 50-100 μg/ml HP under GD insult significantly prevented GD-induced cell death to a higher extent than SNP alone as shown by an augmentation of cell survival and intracellular bcl-2 levels and a decrease of lipid peroxidation, caspase-3 activation and PARP cleavage. Cytoprotection by the NO-donor was almost abolished by the use of a NO scavenger and potentiated by the presence of superoxide dismutase. SNP and/or HP neuroprotection on GD-cells was significantly reversed by rotenone treatment. These results suggest that: (1) SNP could protect PC12 cells from GD-induced cytotoxicity through NO generation and (2) the enhancement of the SNP-mediated neuroprotective effect on GD-cells by HP might arise in part through scavenging of reactive oxygen species (ROS) and inhibition of mitochondrial dysfunction associated with the hypoglycemic episode. This current finding might highlight the development of therapeutic strategies aimed at manipulating NO-donors in combination with herb supplements containing ROS scavenger compounds for prophylaxis from brain ischemia.
Export Options
About this article
Cite this article as:
Maria Munoz, Paloma Bermejo-Bescos, Carmen Romero, Juana Benedi, Sagrario Martin-Aragon , SNP-Mediated Neuroprotection under Glucose Deprivation Is Enhanced by Hypericum perforatum, CNS & Neurological Disorders - Drug Targets 2012; 11 (2) . https://dx.doi.org/10.2174/187152712800269713
DOI https://dx.doi.org/10.2174/187152712800269713 |
Print ISSN 1871-5273 |
Publisher Name Bentham Science Publisher |
Online ISSN 1996-3181 |
Call for Papers in Thematic Issues
Diagnosis and treatment of central nervous system infectious diseases
Infectious diseases of the central nervous system (CNS) can be divided into bacterial, tuberculous, viral, fungal, parasitic infections, etc. Early etiological treatment is often the most crucial means to reduce the mortality rate of patients with central nervous system infections, reduce complications and sequelae, and improve prognosis. The initial clinical ...read more
Techniques of Drug Repurposing: Delivering a new life to Herbs & Drugs
Of late, with the adaptation of innovative approaches and integration of advancements made towards medical sciences as well as the availability of a wide range of tools; several therapeutic challenges are being translated into viable clinical solutions, with a high degree of efficacy, safety, and selectivity. With a better understanding ...read more
Trends and perspectives in the rational management of CNS disorders
Central nervous system (CNS) diseases enforce a significant global health burden, driving ongoing efforts to improve our understanding and effectiveness of therapy. This issue investigates current advances in the discipline, focusing on the understanding as well as therapeutic handling of various CNS diseases. The issue covers a variety of diseases, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Effects of Exposure to Recent Autobiographical Events on Declarative Memory in Amnestic Mild Cognitive Impairment: A Preliminary Pilot Study
Current Alzheimer Research Alternative Approach for Mitigation of Doxorubicin-Induced Cardiotoxicity using Herbal Agents
Current Clinical Pharmacology Beyond Acetylcholinesterase Inhibitors: Novel Cholinergic Treatments for Alzheimer’s Disease
Current Alzheimer Research COVID-19: A Daily Dose of Yoga to Cope with Anxiety
Coronaviruses Role of the Hippocampus and Amygdala in the Extinction of Fear- Motivated Learning
Current Neurovascular Research Structure Conversion and Structure Separation of G-Quadruplexes Investigated by Carbazole Derivatives
Current Pharmaceutical Design Nutrition and Nutraceuticals in Neuroinflammatory and Brain Metabolic Stress: Implications for Neurodegenerative Disorders
CNS & Neurological Disorders - Drug Targets Approaching Clinical Reality: Markers for Monitoring Systemic Inflammation and Sepsis
Current Molecular Medicine The Function of the Selective Inhibitors of Cycloxygenase 2
Mini-Reviews in Medicinal Chemistry Deprescribing: A Psychodynamically-Informed, Patient-Centered Perspective
Current Psychiatry Reviews Editorial (Thematic Issue: Advances in Research on Pharmacological Targets for Pain Relief)
Current Neuropharmacology Mechanical Ventilation of the Neonate: Principles and Strategies
Current Respiratory Medicine Reviews The Effect of Relaxation Technique (Jacobsen and Benson) on Depression, Anxiety, and Stress in Patients with Multiple Sclerosis
Current Psychiatry Research and Reviews Electrochemical Behavior of Melatonin and its Sensing in Pharmaceutical Formulations and in Human Urine
Current Pharmaceutical Analysis A New Series of 1,3-Dimethylxanthine Based Adenosine A<sub>2A</sub> Receptor Antagonists as a Non-Dopaminergic Treatment of Parkinson’s Disease
Current Drug Discovery Technologies A Solvent-free Method for Synthesis of Dihydroangelicins using Microwaves
Current Green Chemistry Using Endogenous Neural Stem Cells to Enhance Recovery from Ischemic Brain Injury
Current Neurovascular Research Terpenes and Phenylpropanoids as Acetyl- and Butyrylcholinesterase Inhibitors: A Comparative Study
Current Alzheimer Research Therapeutic Benefit and Biological Importance of Ginkgetin in the Medicine: Medicinal Importance, Pharmacological Activities and Analytical Aspects
Current Bioactive Compounds Up-Regulation of TLR-4 in the Brain After Ischemic Kidney-Induced Encephalopathy in the Rat
CNS & Neurological Disorders - Drug Targets