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Drug Delivery Letters

Editor-in-Chief

ISSN (Print): 2210-3031
ISSN (Online): 2210-304X

Topical Gene Delivery in Mice Using Gemini Surfactant - Lipid Nanoparticles With and Without Tape Electrode Electroporation

Author(s): I. Badea, N. Shaterian and M. Foldvari

Volume 1, Issue 1, 2011

Page: [62 - 66] Pages: 5

DOI: 10.2174/2210304x11101010062

Abstract

Gemini surfactant-lipid cationic nanoparticles (NP) and electroporation (EP) with a novel tape-electrode were evaluated in mice as topical delivery methods for plasmid DNA encoding luciferase reporter gene or interferon gamma (IFNg). NP treatment of the skin without EP (dose: 25 microgram plasmid DNA/50 microliter formulation) enhanced skin expression of IFNg, three-fold compared to buffer control or topical plasmid solution, whereas application of the noninvasive tape-probe to deliver the electroporative effect after topical NP treatment decreased gene expression compared with topical NP treatment alone. Gemini NPs are a potential topical gene delivery system, however subsequent use of EP showed an inhibitory effect.

Keywords: Electroporation, gene therapy, nanoparticles, non-viral, plasmid, skin, topical, Gemini surfactant-lipid cationic nanoparticles, NP treatment


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