Abstract
Extracellular production of heterologous proteins using the Escherichia coli cell factory offers several advantages over intracellular production and mammalian culture. Properly folded proteins can be rapidly accumulated in the culture media, and downstream processes for isolation and purification can be much simplified. Efforts to enhance the secretory production of target proteins can be largely classified as selection and modification of the signal peptide, coexpression of proteins to assist translocation and folding, improvement of periplasmic release, and protection of target proteins from degradation and contamination. Here, we review recent patents on the secretory production of recombinant proteins in E. coli.
Keywords: Secretion, excretion, periplasm, extracellular production, signal peptide, recombinant protein, Escherichia coli
Recent Patents on Biotechnology
Title: Secretory Production of Recombinant Proteins in Escherichia coli
Volume: 4 Issue: 1
Author(s): Sung Ho Yoon, Seong Keun Kim and Jihyun F. Kim
Affiliation:
Keywords: Secretion, excretion, periplasm, extracellular production, signal peptide, recombinant protein, Escherichia coli
Abstract: Extracellular production of heterologous proteins using the Escherichia coli cell factory offers several advantages over intracellular production and mammalian culture. Properly folded proteins can be rapidly accumulated in the culture media, and downstream processes for isolation and purification can be much simplified. Efforts to enhance the secretory production of target proteins can be largely classified as selection and modification of the signal peptide, coexpression of proteins to assist translocation and folding, improvement of periplasmic release, and protection of target proteins from degradation and contamination. Here, we review recent patents on the secretory production of recombinant proteins in E. coli.
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Cite this article as:
Yoon Ho Sung, Kim Keun Seong and Kim F. Jihyun, Secretory Production of Recombinant Proteins in Escherichia coli, Recent Patents on Biotechnology 2010; 4 (1) . https://dx.doi.org/10.2174/187220810790069550
DOI https://dx.doi.org/10.2174/187220810790069550 |
Print ISSN 1872-2083 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-4012 |
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