Structure-Function Relationship of Vpr: Biological Implications

Nelly      Morellet; Bernard   P.   Roques; Serge      Bouaziz

Abstract

Vpr, incorporated into the HIV-1 virion, shows multiple activities including nuclear transport of the preintegration complex to the nucleus, activation of the transcription, cell cycle arrest at the G2/M transition and induction of apoptosis. Vpr controls many host cell functions through a variety of biological activities and by interaction with cellular biochemical pathways. Nuclear import of Vpr may be due to its interaction with nuclear transport factors and components of the nuclear pore complex. Cell cycle arrest has been correlated with the binding to DCAF1, a cullin 4A-associated factor and apoptosis may be facilitated by interaction with mitochondrial proteins, in a caspase-dependent mechanism. The structure of Vpr(1-96) and various fragments have been determined by NMR in diverse solvents. The different functions of Vpr can be classified according to their relationship with the different structural domains of the protein and appear to correlate with the partners interacting with these domains. Thus, virion packaging seems to be mediated by the first α- helix (17-33), activation of the transcription, regulation of apoptosis and subcellular transport appear to be dependent on the second α-helix (38-50) and cell cycle arrest seems to be induced by the carboxyl terminal α-helix (55-77). Mutational analysis performed by several groups have provided a strong basis to understand the structure-function relationship of Vpr. The aim of this review is to run through these mutations using the available information on sequences and discuss their effect on the functions of Vpr from the point of view of its structure.

Keywords: NMR, HIV, Vpr, mutation, incorporation, nuclear import, cell cycle arrest, transactivation, apoptosis, oligomerisation