Effects of Enzyme Sources on Midazolam 1-Hydroxylation Activity Catalyzed by Recombinant Cytochrome P450 3A4 in Combination with NADPH-Cytochrome P450 Reductase

Title: Effects of Enzyme Sources on Midazolam 1-Hydroxylation Activity Catalyzed by Recombinant Cytochrome P450 3A4 in Combination with NADPH-Cytochrome P450 Reductase

Volume: 2 Issue: 3

Author(s): Chie Emoto, Norie Murayama and Hiroshi Yamazaki

Affiliation:

Keywords: CYP3A4, lipid mixtures, NADPH-P450 reductase, bicistronic expression, E. coli membranes, human

Abstract: The influence of the enzyme source such as phospholipid and expression ratio of NADPHcytochrome P450 reductase on midazolam 1-hydroxylation activity of cytochrome P450 3A4 was investigated in reconstituted systems and in membranes using bicistronic expressions. Preferable ratio of P450 reductase over human P450 3A4 would be a determinant factor for the drug metabolism studies.

Cite this article as:

Emoto Chie, Murayama Norie and Yamazaki Hiroshi, Effects of Enzyme Sources on Midazolam 1-Hydroxylation Activity Catalyzed by Recombinant Cytochrome P450 3A4 in Combination with NADPH-Cytochrome P450 Reductase, Drug Metabolism Letters 2008; 2 (3) . https://dx.doi.org/10.2174/187231208785425737