Comprehensive Identification of Cytochrome P450 Isoforms from Solubility-Based Fractionated Rat Liver Microsomes

Title: Comprehensive Identification of Cytochrome P450 Isoforms from Solubility-Based Fractionated Rat Liver Microsomes

Volume: 1 Issue: 4

Author(s): Hidenori Yamanaka, Masahiro Takeyoshi, Yasushi Minobe, Yoshikuni Yakabe, Mineo Takatsuki and Hisaya Sato

Affiliation:

Keywords: Drug metabolism, membrane proteins, P450 isoforms, rat liver microsome

Abstract: Cytochrome P450 isoforms from male rat liver microsomes were comprehensively identified using nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS). The enrichment of P450, an endomembrane-anchored heme protein, was achieved by solubility-based protein fractionation, and greatly improved the total number of identified P450 isoforms. LC-MS/MS analysis of fractions resulted in the identification of total 36 P450 isoforms. The combination of proteomic analysis and the solubility-based fractionation would provide powerful tool for the expression analysis of the superfamily proteins having great similarities between the amino acids sequences.

Cite this article as:

Yamanaka Hidenori, Takeyoshi Masahiro, Minobe Yasushi, Yakabe Yoshikuni, Takatsuki Mineo and Sato Hisaya, Comprehensive Identification of Cytochrome P450 Isoforms from Solubility-Based Fractionated Rat Liver Microsomes, Drug Metabolism Letters 2007; 1 (4) . https://dx.doi.org/10.2174/187231207783221394